ISO Easy Kits
The ISO Easy Kit is designed to eliminate the tedious and time-consuming process of standardized ISO protocols for the detection and enumeration of somatic and F-specific coliphages.
ISO Easy Kits
The ISO Easy Kit is designed to eliminate the tedious and time-consuming process of standardized ISO protocols for the detection and enumeration of somatic and F-specific coliphages.
Bluephage ISO Easy Kits
Our specially crafted ISO Easy Kits (BP1601 and BP1604) are designed to eliminate the tedious and time-consuming process of the standardized ISO 10705-2 protocol for the detection and enumeration of both somatic and F-specific coliphages.
These kits are an all-in-one kits:
MSA and MSB
Biological material calibrated and ready to use (Strain and positive control included)
Bluephage Easy Kit for Enumeration of Somatic Coliphages
According to ISO 10705-2 – Double Agar Layer (DAL) method
Bacteriophage: Somatic Coliphages
Method: ISO 10705-2
Description
For samples containing large numbers of bacteria.
All types of water: raw and treated wastewater, surface water, recreational water, shellfish extracts, sediments and sludge extract after dilution where necessary.
MSA and MSB ready to use
Host strain (E.coli WG5) and positive control included (ɸX174)
Additional material required and not provided in the Kit:
• MSA powder media sold separately (catalogue no. BP1638)
• Sterile Petri dishes (90mm)
Application
Raw and treated wastewater, surface water, recreational water, shellfish extracts, sediments, and sludge extracts after dilution where necessary.
Sample volume: 1 mL
Number of assays: 70 assays
Product code: BP1601
BP1604
Bluephage Easy Kit for Enumeration of Somatic Coliphages
Based on the ISO 10705-2 – Single Agar Layer (SAL) method
Bacteriophage: Somatic Coliphages
Method: ISO 10705-2 & ISO 10705-2 & US-EPA 1602, 1642 and 1643
Description
This detection and enumeration kit is based on ISO 10705-2 and US-EPA 1602, 1642 and 1643 methods. It contains all the consumables and biological material required to perform the analysis, including freeze-dried specific host-cells for the somatic coliphage group, which are ready for use after 120 min of incubation.
MSA and MSB ready to use
Host strain (WG5) and positive control included (ɸX174)
Additional material required and not provided in the Kit:
• Sterile Petri dishes (140mm)
Application
Samples with expected low counts of bacteriophages (e.g. drinking water, reclaimed water…)
Sample volume: 100 mL
Number of assays: 10 assays
Product code: BP1604
Differences between the standard ISO method and the two Bluephage Easy Kits
| PARAMETER | ISO | Bluephage EASY KIT |
| SENSITIVITY | 1 PFU/1ML | 1 PFU/1ML 1 PFU/100ML |
| EASE TO USE | ∗ | ∗∗ |
| APPLICABILITY | WATER, FOOD, BIOSOLIDS | WATER, FOOD, BIOSOLIDS |
| AVAILABILITY OF STRAINS | NOT INCLUDED | NOT INCLUDED |
| PREPARATION OF MATERIAL (h) (PREVIOUS STEPS) | 40-60 | 0 |
| PREPARATION OF INOCULUM CULTURE (h) | 3-4 | 2 |
| RESULTS TIME (h) | 18-20h | 18-20h |
| HOURS TECHNICIAN | 4 | 1 |
