Rapid Kit for Enumeration of Somatic Coliphages
Rapid Kit for Enumeration of Somatic Coliphages
BPF-SE
ENUMERA® Quantitative Rapid Kit
Bacteriophage: Somatic Coliphages
Description
Kit designed for the specific Enumeration of somatic coliphages in 100 mL of water. Patented technology based on colour change that occurs in the culture medium due to the lysis of the host strain if the somatic coliphages are present in the water sample. Quantification is by Most Probable Number method.
Easy, Express, Efficient, Effective and Electronic are key benefits of Bluephage’s Rapid Kit technology to improve your operations by making processes more cost-effective.
Easy
All-in-one and ready-to-use kits.
Free-agar technology.
No need for overnight plating and manual reading.
Electronic
Bluephage app
Automatic data reading and cloud uploading results.
Effective
High sensitivity and specificity.
Express
20 minutes of hands-on time.
Simplifies and improves the coliphage detection process.
Efficient
Results in record time.
Analysis and detection of coliphages in less than 6,5h. The closest technique for real-time monitoring.
Number of tests
This kit is designed to perform 50 assays with one 100 mL water sample in each one.
Sample volume: 100 mL
Number of assays: 50 assays
Product code: BPF-SE
Bluephage App installed in a mobile device, PC or laptop
Download the “BLUEPHAGE” application for automatic reading of results, printing of final PDF reports, and monitoring of analyses.
LIMS compatible software.
Applications ENUMERA® Quantitative Rapid Kit
This quantitative rapid detection kit is designed for the specific detection and enumeration of somatic coliphages in 100 mL of water. This method applies to all kinds of water and shellfish extracts, sediments, and sludge.
| PARAMETER | EXPECTED CONCENTRATION (PFU/100 ML) | ANALYSIS VOLUME | RAPID KITS |
|---|---|---|---|
| Somatic coliphages Somatic coliphages 0 100 mL BPF-SE (Quantitative) | 0 | 100 mL | BPF-SE (Quantitative) |
| PARAMETER | EXPECTED CONCENTRATION (PFU/100 ML) | ANALYSIS VOLUME | RAPID KITS |
|---|---|---|---|
| Somatic coliphages Somatic coliphages 104-106 1 mL BPF-SE (Quantitative) | 104-106 | 1 mL | BPF-SE (Quantitative) |
| PARAMETER | EXPECTED CONCENTRATION (PFU/100 ML) | ANALYSIS VOLUME | RAPID KITS |
|---|---|---|---|
| Somatic coliphages Somatic coliphages 102-104 1 mL BPF-SE (Quantitative) | 102-104 | 1 mL | BPF-SE (Quantitative) |
| PARAMETER | EXPECTED CONCENTRATION (PFU/100 ML) | ANALYSIS VOLUME | RAPID KITS |
|---|---|---|---|
| Somatic coliphages Somatic coliphages 0-103 1-100 mL BPF-SE (Quantitative) | 0-103 | 1-100 mL | BPF-SE (Quantitative) |
| PARAMETER | EXPECTED CONCENTRATION (PFU/100 ML) | ANALYSIS VOLUME | RAPID KITS |
|---|---|---|---|
| Somatic coliphages Somatic coliphages 0-104 1-100 mL BPF-SE (Quantitative) | 0-104 | 1-100 mL | BPF-SE (Quantitative) |
What is contained in the ENUMERA® Quantitative Rapid Kit
• 50 vials with Escherichia coli CB10® host strain
• 50 BP Testing bottles with culture media
• 50 ENUMERA® Testing trays for quantification by MPN
• 10 vials with Bacteriophage positive control
Equipment not included
• Incubator or water bath thermostatically controlled at (36±2)°C and (30±2)°C.
• ENUMERA® TERMO SEALER RPS-H2O (compatible with other termo-sealer)
• Mobile device PC or laptop with Bluephage APP
How to perform the coliphage test with the ENUMERA® Quantitative Rapid Kit
1. Preparing the sample
2. Preparing the host strain
3. Measure 100 mL of the water sample
4. Mixing the resuspended host strain and the sample
5. Starting the test. Resuspension of Bluephage Medium and filling of ENUMERA® Testing Tray.
6. Sealing the ENUMERA® Testing Tray with ENUMERA® SEALER RPS-H2O.
7. Running the test
8. Reading the results
The time to results is 6.5 hours when starting the ENUMERA®
| Hands-on time | < 20 min |
| Test time | < 6.5 h |
| Incubation conditions | 1h at (36±2)ºC 5.5h at (30±2)ºC |
| Shelf Life | 1 year |
| Storage conditions | Biological Material vials (E.coli & positive control) at -20 ºC BP Testing bottles (culture media) at room temperature |
BLUEPHAGE validation demonstrates that the BPF-SE kit presents a detection level equivalent to the ISO 10705-2 method. This detection level represents the minimum theoretical concentration of microorganisms required to yield observable growth with a probability of P = 0.95 when introduced into a specific culture medium and subjected to controlled incubation conditions, as specified in the ISO 16140-1 and ISO 13843 methods.
The BPF-SE kit detects somatic coliphages at 1 PFU/100 mL.
